Journal: Microbial Biotechnology

Article Title: A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers

doi: 10.1111/1751-7915.70292

Figure Lengend Snippet: The prevalence of Fusobacterium in Southern Chinese population with correlations to host conditions. (A) The prevalence of Fusobacterium in Southern Chinese population; (B) The prevalence of Fusobacterium species and (C) subspecies in Southern Chinese population; (D) The correlations between Fusobacterium and host parameters. The correlation analysis is spearman analysis. A higher absolute value of the correlation coefficient signifies a stronger correlation, with red denoting a positive correlation and blue indicating a negative correlation. The size of the nodes and the depth of their colour represent the strength of the correlation, where larger nodes and darker hues correspond to stronger correlations. An asterisk (*) denotes the p ‐value. FM, Fusobacterium mortiferum ; FN, Fusobacterium nucleatum ; FNA, Fusobacterium nucleatum subsp. animalis ; FNE, Fusobacterium necrophorum .; FNN, Fusobacterium nucleatum subsp. nucleatum ; FNP, Fusobacterium nucleatum subsp. polymorphum ; FNV, Fusobacterium nucleatum subsp. vincentii ; FU, Fusobacterium ulcerans ; FV, Fusobacterium varium .

Article Snippet: 4 , F. necrophorum , ATCC 25286 , − , + , −.

Techniques:

Journal: Microbial Biotechnology

Article Title: A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers

doi: 10.1111/1751-7915.70292

Figure Lengend Snippet: The prevalence of Fusobacterium in faeces of cancer patients with correlations to host conditions. (A) The prevalence of Fusobacterium in cancer patients ( n = 254) and healthy controls ( n = 82); (B–E) The prevalence of Fusobacterium species and subspecies in cancer patients ( n = 254) and healthy controls ( n = 82); (F–N) The relative abundance of FN (F), FNN (G), FNA (H), FNV (I), FNP (J), FM (K), FU (L), FV (M) and FNE (N) in faecal samples of cancer patients ( n = 254) and healthy controls ( n = 82). Mann–Whitney test. (O) The correlations between Fusobacterium and host parameters in cancer patients ( n = 254). The correlation analysis is spearman analysis. A higher absolute value of the correlation coefficient signifies a stronger correlation, with red denoting a positive correlation and blue indicating a negative correlation. The size of the nodes and the depth of their colour represent the strength of the correlation, where larger nodes and darker hues correspond to stronger correlations. An asterisk (*) denotes the p ‐value. (P) The correlations between Fusobacterium and host parameters in healthy controls ( n = 82). The correlation analysis is spearman analysis. A higher absolute value of the correlation coefficient signifies a stronger correlation, with red denoting a positive correlation and blue indicating a negative correlation. The size of the nodes and the depth of their colour represent the strength of the correlation, where larger nodes and darker hues correspond to stronger correlations. An asterisk (*) denotes the p ‐value. (Q) The receiver operating characteristic (ROC) curve for predicting cancers. AUC, the area under the ROC curve. The 95% confidence intervals are shown in brackets. (R) The prevalence of Fusobacterium in untreated ( n = 64), treated (190) and control ( n = 82) group. (S, T) The prevalence of Fusobacterium species and subspecies in untreated ( n = 64), treated (190) and control ( n = 82) group. (U–AC) The relative abundance of FN (U), FNN (V), FNA (W), FNV (X), FNP (Y), FM (Z), FU (AA), FV (AB) and FNE (AC) in faecal samples of untreated ( n = 64) and treated ( n = 190) cancer patients and control group ( n = 82). Kruskal–Wallis H test followed by Dunn's test. FM, Fusobacterium mortiferum ; FN, Fusobacterium nucleatum ; FNA, Fusobacterium nucleatum subsp. animalis ; FNE, Fusobacterium necrophorum .; FNN, Fusobacterium nucleatum subsp. nucleatum ; FNP, Fusobacterium nucleatum subsp. polymorphum ; FNV, Fusobacterium nucleatum subsp. vincentii ; FU, Fusobacterium ulcerans ; FV, Fusobacterium varium .

Article Snippet: 4 , F. necrophorum , ATCC 25286 , − , + , −.

Techniques: MANN-WHITNEY, Control

Journal: Microbial Biotechnology

Article Title: A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers

doi: 10.1111/1751-7915.70292

Figure Lengend Snippet: The compositional features of Fusobacterium communities in colorectal cancer tissues. (A) The positive detection rate of Fusobacterium in tumour tissues ( n = 15), adjacent normal tissues ( n = 15) and normal tissues ( n = 16). (B‐E) The prevalence of Fusobacterium species and subspecies in tumour tissues ( n = 15), adjacent normal tissues ( n = 15) and normal tissues ( n = 16). (F–N) The relative abundance of FN (F), FNN (G), FNA (H), FNV (I), FNP (J), FM (K), FU (L), FV (M) and FNE (N) in tumour ( n = 15), adjacent normal ( n = 15) and normal tissues ( n = 16). Kruskal–Wallis H test followed by Dunn's test. (O) The detection of Fusobacterium in tumour ( n = 15), adjacent normal ( n = 15) and normal tissues ( n = 15). Their relative quantitation in the Fusobacterium community of each sample are presented as a heatmap. The relative abundance of Fusobacterium was depicted using a colour scale, where red denoted high relative abundance and blue signified low relative abundance. FM, Fusobacterium mortiferum ; FN, Fusobacterium nucleatum ; FNA, Fusobacterium nucleatum subsp. animalis ; FNE, Fusobacterium necrophorum .; FNN, Fusobacterium nucleatum subsp. nucleatum ; FNP, Fusobacterium nucleatum subsp. polymorphum ; FNV, Fusobacterium nucleatum subsp. vincentii ; FU, Fusobacterium ulcerans ; FV, Fusobacterium varium .

Article Snippet: 4 , F. necrophorum , ATCC 25286 , − , + , −.

Techniques: Quantitation Assay

Journal: Microbial Biotechnology

Article Title: A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers

doi: 10.1111/1751-7915.70292

Figure Lengend Snippet: The identification of Fusobacterium in paired tumour tissues and faeces of lung cancer patients. (A, B) The prevalence of Fusobacterium (A) and Fusobacterium species (B) in paired tumour tissue ( n = 46), adjacent normal tissue ( n = 46), normal tissue ( n = 45) and faeces ( n = 48) of lung cancer patients. (C, D) The relative abundance of FN (C) and FU (D) in tumour tissue ( n = 46), adjacent normal tissue ( n = 46) and normal tissue ( n = 45) of lung cancer patients. Kruskal–Wallis H test followed by Dunn's test. (E) The correlations between faecel Fusobacterium and host parameters ( n = 48). The correlation analysis is spearman analysis. A higher absolute value of the correlation coefficient signifies a stronger correlation, with red denoting a positive correlation and blue indicating a negative correlation. The size of the nodes and the depth of their colour represent the strength of the correlation, where larger nodes and darker hues correspond to stronger correlations. An asterisk (*) denotes the p ‐value. (F) The detection of Fusobacterium in tumour tissue ( n = 46), adjacent normal tissue ( n = 46) and normal tissue ( n = 45) of lung cancer patients. Their relative quantitation in the Fusobacterium community of each sample are presented as a heatmap. The relative abundance of Fusobacterium was depicted using a colour scale, where red denoted high relative abundance and blue signified low relative abundance. FM, Fusobacterium mortiferum ; FN, Fusobacterium nucleatum ; FNA, Fusobacterium nucleatum subsp. animalis ; FNE, Fusobacterium necrophorum .; FNN, Fusobacterium nucleatum subsp. nucleatum ; FNP, Fusobacterium nucleatum subsp. polymorphum ; FNV, Fusobacterium nucleatum subsp. vincentii ; FU, Fusobacterium ulcerans ; FV, Fusobacterium varium .

Article Snippet: 4 , F. necrophorum , ATCC 25286 , − , + , −.

Techniques: Quantitation Assay

Journal: Microorganisms

Article Title: Characterization of Three New Outer Membrane Adhesion Proteins in Fusobacterium necrophorum

doi: 10.3390/microorganisms11122968

Figure Lengend Snippet: Rabbit polyclonal antibodies to recombinant OMPs inhibit adhesion of F. necrophorum to bovine endothelial (EJG) cells. Adhesion inhibition assays with F. necrophorum and EJG cells using rabbit (1:100) polyclonal antibodies raised against ( A ) r43 kDa OMP; ( B ) rOmpH and rOmpA; ( C ) rOmpH, rOmpA, and rCSP; and ( D ) rOmpH, rOmpA, rCSP, and r43 kDa OMP. Significance was determined by unpaired two-tailed Student’s t -test, n = 3; * p < 0.05, ** p <0.01, *** p < 0.001, and **** p < 0.0001. The bars indicate the mean ± standard error of the mean (SEM) of data pooled from triplicate experiments. ( E ) Giemsa staining of EJG cells following adhesion inhibition assays: ( i ) uninfected (negative control), ( ii ) infected with F. necrophorum (positive control), and ( iii ) infected with F. necrophorum pre-treated with a combination of four polyclonal antibodies, as in panel ( D ).

Article Snippet: OMP proteins were identified by Western blot using a polyclonal antibody raised against F. necrophorum subsp. necrophorum OMPs as the primary antibody [ ] (Cocalico Biologicals, Stevens, PA, USA) and an alkaline phosphatase-conjugated anti-rabbit secondary antibody (12-448, EMD Millipore Corp, Billerica, MA, USA).

Techniques: Recombinant, Inhibition, Two Tailed Test, Staining, Negative Control, Infection, Positive Control